Identification of testicular atypical germ cells by an immunohistochemical technique for placental alkaline phosphatase
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چکیده
منابع مشابه
Mutation of a single amino acid converts germ cell alkaline phosphatase to placental alkaline phosphatase.
Human placental and germ cell alkaline phosphatases (PLAP and GCAP, respectively), are characterized by their differential sensitivities to inhibition by L-leucine, EDTA, and heat. Yet, they differ by only 7 amino acids at positions 15, 67, 68, 84, 241, 254, and 429 within their respective 484 residues. To determine the structural basis and the amino acid(s) involved in these physicochemical di...
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proliferative disorders. It is necessary to accurately identify the megakaryocytic cells in order to diagnose megakaryocytic leukemia and to assess the significance of the pres ence of such cells in the circulating blood . At present, megakaryocytes and their precursors can be best identified either by ultrastructural study of platelet peroxidase or by immunocytochemical studies using monoclona...
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This concise review summarises tissue and serum markers useful for differential diagnosis of germ cell tumours (GCT), with focus on the most common testicular GCT (TGCT). GCT are characterised by phenotypic heterogeneity due to largely retained embryonic pluripotency and aberrant somatic differentiation. TGCT that occur in young men are divided into two main types, seminoma and nonseminoma, bot...
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Wheat germ agglutinin (WGA) precipitates bone type serum alkaline phosphatase (sALP) isoenzyme specifically. The precipitates are composed of the macromolecules of WGA and "bone type sALP" (WGA-ALP complex). In order to use bone type sALP as a marker in polyacrylamide gel electrophoresis (PAGE), a method to separate "bone type sALP" from the "WGA-ALP complex" was established by using N-acetyl-D...
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Placental alkaline phosphatase (placental ALP, PLAP) and germ-cell ALP (GCAP, also known as placental-like ALP), expressed in gonadal cancer tissues, are potential tumor markers. Four monoclonal antibodies, raised against PLAP and recognizing different epitopes, were selected to study the influence of the following variables on the accuracy of PLAP and GCAP measurement: phenotype, molecular for...
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ژورنال
عنوان ژورنال: Cancer
سال: 1987
ISSN: 0008-543X,1097-0142
DOI: 10.1002/1097-0142(19870915)60:6<1325::aid-cncr2820600627>3.0.co;2-p